![]() ![]() We realize that not all potential KM-Express users will already have candidate biomarker gene(s) and thus may want to identify their own using the datasets archived in KM-Express. To help users better understand the clinical or pathological terms, interpretive statements are shown along with the boxplot. After the job has been submitted and the analysis is finished, the result of the expression comparison for the input gene will be shown as a boxplot and will be catego- rized according to the clinical or pathological information based on the study. In the 'Analysis' panel of the homepage, users type in the gene symbol of the gene(s) they are interested in and then select the cancer type and a dataset from either patients or cell lines. expression analysis KM-Express is also designed to perform gene expression analysis based on patients and cell line datasets (Figure 3). The significance P-value and hazard ratio are provided in the figure along with the summary of input genes and user-selected parameters. (3) The output provides KM-plot figures in both PDF and PNG format. For Q3Q1, highly expressed group represents patients with input gene expression >75% patients, while the lowly expressed group represents patients with input gene expression <25% patients. For median/average/25%/75% cutoff, higher than the threshold corresponds to highly express group, others are lowly express group. Next, select the criteria to divide the patients into two groups, one group highly expressing the input gene, the other group lowly expressing the input gene. ![]() The survival outcome is calculated as biochemical recurrence for tumor or without tumor for prostate cancer and dead or alive status for breast cancer. After the cancer type is selected, the RNA-seq data available for survival analysis will be shown in 'Database' panel. The expression level of the multiple genes will be averaged and used for survival analysis. When users input multiple genes, gene symbols should be separated by ','. (1) Enter the gene symbol in the box provided. Step-by-step usage of KM-Express for survival analysis. ![]()
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